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Nadim Majdalani

NIH/NCI

Laboratory of Molecular Biology

37 Convent Drive

Bldg 37, Rm 5132

Bethesda, MD 20892-4264

E-mail: nadim@nih.gov

Phone: (301) 451-8788

FAX: (301) 496-2212


Date and Place of Birth: March 3, 1964, Beirut, Lebanon


Nationality: US Citizen


Education and training


2003- Staff Scientist. Dr. Susan Gottesman, National Institutes of Health, National Cancer Institute. Small RNAs as gene regulators in E. coli.


    1. Research Fellow. Dr. Susan Gottesman, National Institutes of Health, National Cancer Institute. Isolation and characterization of a novel small RNA, RprA.




    1. Post-doctoral fellow. Dr. Susan Gottesman, National Institutes of Health, National Cancer Institute. (Fogarty Visiting fellow 1995-1997, CRTA post-doctoral fellow 1997- ). Characterization of the translational and transcriptional regulation activities of the small RNA, DsrA.


1988-1995 Graduate Research Assistant, Dr. Karin Ippen-Ihler, Medical Microbiology and Immunology, Texas A&M University Health Sciences Center.

Thesis: Maturation of F propilin: the roles of the propilin leader peptide, host encoded proteins, and the F transfer protein TraQ.

Ph.D. Texas A&M University, 1995.


1986-1988 Graduate Research Assistant, Dr. Allison Ficht, Medical Biochemistry, Texas A&M University. Isolation and collection of ^ Fasciola hepatica organisms from cattle liver. Screening and characterization of eggshell antigens.


1985-1986 Graduate Teaching Assistant, Biology Department, American University of Beirut.


1982-1985 Bachelor of Science, American University of Beirut.


Miscellaneous


American Society for Microbiology member

Lambda Lunch Procaryote Interest group member

NIH Radiation Safety Authorized User

Building 37 Emergency and Evacuation Floor Team Coordinator

Article reviewer for the Journal of Bacteriology, the American Journal of PharmacoGenomics, RNA biology, FEMS Letters, Genetics and Molecular Microbiology.


^ External reviewer. Graduate student thesis, Manuel Saavedra De Bast, Chromosomal Toxin-Antitoxin Systems May Acting as Antiaddiction Modules. Feb 27, 2009 Free University of Brussels, Gosselies, Belgium.


Collaborations:

Sarah Woodson/Toby Soper, Johns Hopkins University, In Vivo analysis of the rpoS mRNA leader recruitment of Hfq to facilitate annealing with DsrA sRNA.


Magnus Essand/Di Yu, Uppsala University. Developing a recombineering system to genetically engineer the Adenoviral genome.


Teaching and mentoring


Yodit Tsegaye Summer Intern 2010. Screen and isolation for IraM-independent RssB mutants. University of Maryland sophomore.


Mariba Ferguson Summer Intern 2009. Isolation of gain of function RssB mutants. High School graduate/University of Maryland rising freshman.


Mariba Ferguson Intern 2008-2009. Structure function studies of RssB. Eleanor Roosvelt High School senior.


Jessica Mann Summer intern 2007. Undergraduate scholarship program. Structure function studies of RssB. Juniata College, currently graduate student UMDNJ, the State University of New Jersey Medical School, Newark, NJ.


Jihanne Benhammou Post-Bac fellow 2005-2006. Undergraduate Scholarship Program. Mechanism of RprA regulation of RpoS mRNA stability. University of Texas, Austin.


Jihanne Benhammou Summer Intern, 2004. Undergraduate Scholarship Program. Mechanism of RprA regulation of RpoS mRNA stability. Junior, University of Texas, Austin.


Meredith Moore Intern, 2002-2003 National Institutes of Health. Characterization of EmrR, a Mar-like gene regulator. Walt Whitman High School Junior.


Brandon Ogbunugafor Undergraduate minority scholar. 2000- . Isolation of targets of RprA activity. Chemistry senior, Howard University, Washington, D.C.


David Hernandez Undergraduate Scholarship program, pre-CRTA Fellow, 1999-2000; National Institutes of Health. Analysis of the promoter of RprA, a novel small RNA that regulates RpoS translation. Graduate InterAmerican University, Puerto Rico.


Kristin St John Intern, 1998-1999; National Institutes of Health. Characterization of a new RNA with DsrA-like activity. Walt Whitman High School senior.


Jonathan Murrow Summer intern, 1998; National Institutes of Health. Characterization of a set of overlapping clones with DsrA-like activity. Emory University School of Medicine.


SuAnn Chen Summer intern, 1997; National Institutes of Health. Screening and isolation of DsrA-like clones from an E. coli library. Princeton University.


Anirban Gupta Summer intern, 1996; National Institutes of Health. Temperature regulation of the small RNA, DsrA. University of Maryland.


Catherine Ward Honors program senior project; 1993-1994; Texas A&M University Dept. of Biochemistry. Winner of best Biochemical Thesis Award, Honors Program in Biochemistry. Construction of TraQ-PhoA fusions to determine the membrane topology of TraQ. Currently, Med III University of Texas Medical Branch, Galveston, Texas.


Trang Nguyen Summer intern, 1994; Texas A&M University. Characterization of TraA pilin. Texas A&M School of Medicine.


Justin Cetas Summer intern; 1993; Texas A&M University. Over-expression and purification of TraQ. University of Arizona.


1988-1989 Teaching Assistant, Introductory Biology, Texas A&M University.


1986-1987 Teaching Assistant, Introductory Biology, Texas A&M University.


1985-1986 Teaching Assistant, Botany, American University of Beirut.


Awards


NIH Fellows Award for Research Excellence 1999. National Institutes of Health, Bethesda, MD. October 19, 1998.


1st Prize Award poster contest. Graduate Research Symposium. Texas A&M University College of Medicine, College Station, TX. March 9, 1995.


Invited Research Seminars


Multilevel regulation of the E. coli stress and stationary-phase transcription factor RpoS. American University Medical Center, Dept. of Biochemistry, Beirut, Lebanon November 9, 2007


Regulatory small RNAs and their role in the physiology and adaptation of E.coli. The American Univeristy of Beirut, Dept. Biology, Beirut Lebanon, January 24, 2007.


Small RNA regulation of gene expression in ^ E. coli. The Free University of Brussels, Brussels, Belgium. October 11, 2006


Gene Regulation by small regulatory RNAs in E. coli. The Pasteur Institute, Paris, France. October 9, 2006


Non-coding RNA regulation of gene expression in E. coli. Rudbeck Laboratory, University of Uppsala, Sweden. October 5, 2006


Regulation of the small RNA modulators of a developmental switch in ^ E. coli. NHBLI, National Institutes of Health, Bethesda, MD, April 22, 2005.


Regulation by small RNAs; what we have learned from E. coli. Biology Dept., American University of Beirut, Beirut, Lebanon, January 7, 2005.


Stealth regulation: the use of small RNAs in gene regulation in E. coli. Biology Dept., American University of Beirut, Beirut, Lebanon, January 3, 2003.


Régulation de l'expression génétique par les petits ARNs: de l'antisens à l'interférence. Ce que les bactéries nous ont appris. Dept. of Microbiology, IBMM Free University of Brussels, Gosselies, Belgium, December 16, 2002.


Stealth regulation: the use of small RNAs in gene regulation in E. coli. Dept. of Biochemistry, IBPC, Paris, France, December 15, 2002.


Translational regulation of RpoS in response to the environment is mediated by at least two small regulatory RNAs: DsrA and RprA. Division of Genetics, Texas A&M University, College Station, TX, January 17, 2002.


The response of E. coli to certain environmental conditions is mediated by two small regulatory RNAs: DsrA and RprA. Biology Department, The American University of Beirut, Beirut, Lebanon. July 19, 2001.


Environmental sensing of RpoS is mediated by DsrA and RprA: the mode of action and the regulation of synthesis of RprA. The Lambda lunch series, Procaryote Interest Group, National Institutes of Health, June 28, 2001.


The translational regulation of RpoS in response to some environmental signals is mediated by two small RNAs: DsrA and RprA. FASEB Summer Research Conference on Post-Transcriptional Control of Gene Expression; Copper Mountain, CO, July 19, 2000.


Functional small RNAs: DsrA and RprA as activators of RpoS-lacZ translation. The Lambda lunch series, Procaryote Interest Group, National Institutes of Health, December 2, 1999.


DsrA RNA regulates RpoS translation and acts as an antisilencer of transcription of H-NS-silenced genes. The Pasteur Institute, Paris, France. July 9, 1998.


DsrA RNA regulates the translation of the stationary-phase sigma factor RpoS by an anti-antisense mechanism. The Free University of Brussels, Belgium, June 22, 1998.


Transcrptional and translational regulation functions of the small RNA, DsrA. The Lambda lunch series, Procaryotic interest group, National Institutes of Health, May 1, 1997.


Maturation of F-pilin. The Lambda lunch series, Procaryotic interest group, National Institutes of Health, December 9, 1995.

Research Abstracts


McCullen, C. Benhammou, J. Majdalani, N. and S. Gottesman. Regulation of the general stress response transcription factor, RpoS, by small, non-coding RNAs. Molecular Genetics of Bacteria and Phages. Cold Spring Harbor Laboratory, NY, Aug. 22-26, 2006.


Majdalani, N. and S. Gottesman. Signaling through the Rcs phosphorelay. Microbial Genetics and Genomics III. Moab, UT, May 6-9, 2005.


Majdalani, N., and S. Gottesman. Signal transduction in the Rcs phospho-relay pathway. Molecular Genetics of Bateria and Phages. Madison, WI, Aug. 24-29, 2004.


Majdalani, N, M. Heck, D. Hernandez D., S. Gottesman and V. Stout. Components of the RcsB/RcsC phospho-relay pathway. Amer. Soc. for Microbiology 103rd General meeting. Washington D.C., May 18-22, 2003.


Majdalani, N, D. Hernandez and S. Gottesman. Regulation and mode of action of RprA, a small RNA that stimulates RpoS translation. Amer. Soc. for Microbiology 102nd General meeting. Salt Lake City, UT, May 19-23, 2002.


Majdalani, N, D. Hernandez and S. Gottesman. Environmental signals regulate RpoS expression via two small RNAs: DsrA and RprA. Genetics and Genomics of Microbial Systems. Fontevraud l’Abbayye, France, April 12-15, 2002.


Majdalani, N, D. Hernandez and S. Gottesman. The synthesis of the RpoS regulator, RprA, is controlled by RcsB and the capsule two component regulation system RcsB/C. Amer. Soc. Microbiology 101st General meeting, Orlando, FL, May 20-24, 2001.


Majdalani, N, D. Sledjeski, F. Repoila, C. Cunning, T. Elliott, SA. Chen, J Morrow, K. St John, D. Hernandez and S. Gottesman. The translational regulation of RpoS in response to some environmental signals is mediated by two small RNAs: DsrA and RprA. FASEB Summer Research Conference on Post-Transcriptional Control of Gene Expression; Copper Mountain, CO, July 16-21, 2000.


Majdalani N, J. Murrow, S. Chen, K. St John and Gottesman S. Functional small RNAs: a potential small RNA that can promote the expression of an RpoS-lacZ translational fusion. Amer. Soc. Microbiology 99th General meeting, Chicago, IL, May 30-June 3, 1999.


Majdalani N, C. Cunning, T. Elliott, D. Sledjeski, K. St John, SA. Chen, J. Murrow and Gottesman S. Small regulatory RNAs: the DsrA and RprA stories. Molecular genetics of microbial systems, Amalfi, Italy, April 9-12, 1999.


Majdalani N, Cunning C, Elliott T and Gottesman S. Regulation of RpoS translation by DsrA RNA: an anti-antisense model. NIH Research Festival, Bethesda, MD, October 6-9, 1998.


Majdalani N, Moore D, Mannewannakul S, Ward C and Ippen-Ihler K. Partial characterization of the TraQ protein of the conjugative plasmid F. Fallen Leaf Lake Conference on Unifying Themes Among Protein and Nucleic Acid Transporters of Bacteria. Fallen Leaf Lake, South Lake Tahoe, California, September 17-20, 1998.


Majdalani N, Sledjeski D, Cunning C, Elliott T and Gottesman S. Independent transcriptional and translational effects of a small RNA. Cold Spring Harbor Symposium on Mechanisms of transcription. Cold Spring Harbor, New York, June 3-8, 1998.


Majdalani N, Cunning C, Elliott T and Gottesman S. Regulation of RpoS translation by DsrA RNA: an anti-antisense model. Amer. Soc. Microbiology 98th General meeting, Atlanta, Ga, May 17-21, 1998.


Majdalani, Sledjeski and Gottesman. Independent regulation by the small RNA, DsrA, of H-NS silencing of transcription and RpoS translation in E. coli. Keystone Symposia on Molecular and Cellular Biology. The Bacterial Chromosomes meeting, Santa Fe, New Mexico, February 6-12, 1998.


Majdalani, Sledjeski and Gottesman. The small RNA, DsrA, regulates translation of the stationary phase sigma factor RpoS and transcription of H-NS-silenced genes by different pathways. Amer. Soc. Microbiology 97th General Meeting, Miami, Fla May 4-8, 1997.


Majdalani and Ippen-Ihler. Translocation of F-pilin. Genetics of regulation, protein export and cell division. The Jonathan Beckwith Reunion Meeting, Woods Hole, MA, April 19-21, 1996.


Majdalani and Ippen-Ihler. Maturation of F-pilin is sec-independent, TraQ-dependent, and is inhibited by a temperature-sensitive mutation affecting leader peptidase. Cold Spring Harbor Symposium on Quantitative Biology. Protein kinesis: the dynamics of protein trafficking and stability. Cold Spring Harbor Laboratory, NY. May 31-June 7, 1995.


Majdalani, Maneewannakul, Moore and Ippen-Ihler. Processing of the F-pilin precursor, propilin. Amer. Soc. Microbiol. 95th General Meeting, Washington, DC. May 21-25, 1995.


Majdalani and Ippen-Ihler. Maturation of F-pilin. Graduate Research Symposium. Texas A&M University College of Medicine, College Station, TX. March 9-10, 1995.


Majdalani and Ippen-Ihler. Maturation of F-pilin is secA-independent but is inhibited by a temperature-sensitive mutation affecting LepB. Amer. Soc. Microbiol. 93rd General Meeting, Atlanta, Ga. May 16-20, 1993.


Majdalani and Ippen-Ihler. Maturation of F-pilin is SecA-independent but is inhibited by a temperature-sensitive mutation affecting LepB. The Beckwith Reunion Meeting, Woods Hole, MA; March 19-21, 1993.


Publications


Benhammou, J., Majdalani, N. and S. Gottesman. Positively regulated targets of the small RNA, RprA. (Manuscript in preparation).


Majdalani, N. and S. Gottesman. Signaling from RcsF through RcsC. (Manuscript in preparation).


Battesti, A, Majdalani, N, Gottesman, S.: The RpoS General Stress Response: Complexity in Mechanisms for Control and Output. Annual Reviews in Microbiology, Vol. 65, 2011


McCullen, C., Benhammou, J., Majdalani, N. and S. Gottesman (2010). Mechanism of Positive Regulation by DsrA and RprA sRNAs: Pairing increases Translation and Protects rpoS mRNA From Degradation. J. Bact. 192: 5559-71.


Soper T, Mandin P, Majdalani N, Gottesman S, Woodson SA (2010) Positive regulation by small RNAs and the role of Hfq. Proc Natl Acad Sci USA 107: 9602–9607.


Majdalani, N. and S. Gottesman. (2007). Genetic Dissection of signaling through the Rcs phosphorelay. Methods of Enzymology Vol.423 pp. 349-62.


Gottesman, S., N. Majdalani J. Benhammou, C. McCullen, M. Guillier, K. Thompson, C. Vanderpool, N. Sowa, D. FitzGerald and Peter FitzGerald. (2006). Small RNA Regulators and the Bacterial Response to Stress. Cold Spring Harbor Symposia on Quantitative Biology. Vol. LXXI, pp 1-12.


Majdalani, N., M. Heck, V. Stout and S. Gottesman. (2005). Role of RcsF in signaling to the Rcs phosphorelay in Escherichia coli. J. Bact. 187(19) 6770-6778.


Majdalani, N. and S. Gottesman. (2005). The Rcs Phosphorelay: a complex signal transduction system. Annual Reviews in Microbiology. Vol. 59 pp. 379-405.


Majdalani, N., C. Vanderpool and S. Gottesman. (2005) Bacterial small RNA regulators. Critical Reviews in Biochemistry and Molecular Biology. 40(2) 93-111.


Majdalani, N. and S. Gottesman. (2004). Below our radar: Small RNAs are stealthy regulators of gene expression. CCR Frontiers in Science (3) 4-5.


Majdalani, N., F. Repoila and Gottesman, S. (2003). Small non-coding RNAs, coordinators of adaptation processes in Escherichia coli: The RpoS paradigm. Mol. Microbiol. 48 (4) 855-861.


Massé, E., N. Majdalani and Gottesman, S. (2003). Regulatory roles for small RNAs in Bacteria. Current Opinion in Microbiology (6) 1-6.


Majdalani N, D. Hernandez and Gottesman, S. (2002). Regulation and mode of action of the second small RNA activator of RpoS translation, RprA. Mol. Microbiol 46 (3) 813-826.


Gottesman S, Storz G, Rosenow C, Majdalani N, Repoila F, Wassarman K. (2001). Small RNA Regulators of Translation: Mechanisms of Action and Approaches for Identifying New Small RNAs. Cold Spring Harbor Symposia on Quantitative Biology: The Ribosome, Vol. 66 pp 353-362.


Majdalani N, Chen SA, Murrow J, St John K, Gottesman S. (2001). Regulation of RpoS by a novel small RNA: the characterization of RprA. Mol. Micro. 39(5) 1382-1394.


Majdalani N, Sledjeski D, Cunning C, Elliott T, Gottesman S (1998). DsrA regulates translation of RpoS by an anti-antisense mechanism, independent of its action as an antisilencer of transcription. Proc. Natl. Acad. Sci. USA 95 (21) 12462-12467.


Gottesman S, Majdalani N, Sledjeski D (1997). Transcriptional and translational control by DsrA, a small RNA. FASEB JOURNAL 11: (9) 75 , Suppl. S JUL 31.


Majdalani N, Maneewannakul S, Moore D, Ippen-Ihler K (1996). Role of the propilin leader peptide in the maturation of F-pilin. J. Bact. 178 (13) 3748-3754.


Majdalani N, Moore D, Ippen-Ihler K (1996). Membrane insertion of the F-pilin subunit is Sec-independent but requires leader peptidase B and the proton motive force. J. Bact. 178 (13) 3742-3747.


Majdalani N, Ward C, Moore D, Ippen-Ihler K. Topology of the F-transfer protein TraQ (manuscript in preparation).


Majdalani N, Moore D, Maneewannakul S, Ippen-Ihler K. The F-transfer protein TraQ acts as a chaperone to promote translocation of the F-transfer pilin subunit (manuscript in preparation).





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